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Recent Studies on the Characterization of Anaplasma marginale Isolated from North American Bison
aDepartment of Veterinary Pathobiology, College of Veterinary Medicine, cDepartment of Zoology and Collection of Vertebrates, Oklahoma State University, Stillwater, Oklahoma, USA bCanadian Food Inspection Agency, Lethbridge Laboratory, Animal Diseases Research Institute, Lethbridge, Alberta, Canada, dThe Nature Conservancy, Tallgrass Prairie Preserve, Pawhuska, Oklahoma, USA
Address for correspondence: Katherine M. Kocan, Department of Veterinary Pathobiology, College of Veterinary Medicine, 250 McElroy Hall, Oklahoma State University, Stillwater, OK 74078-2007, USA. Voice: (405) 744-7271; fax: (405) 744-5275. kmk285{at}cvm.okstate.edu
Anaplasma marginale (Rickettsiales: Anaplasmataceae), a tick-borne pathogen of cattle, is endemic in tropical and subtropical regions of the world. Many geographic isolates of A. marginale occur worldwide that have been identified by major surface protein (MSP) 1a, which varies in sequence and molecular weight owing to different numbers of tandem 28-29 amino acid repeats. Although serologic tests have identified American bison, Bison bison, as being infected with A. marginale, the present studies were undertaken to confirm A. marginale infection in bison, to characterize bison isolates, and to compare the phylogenetic relationship of the bison isolates with other A. marginale isolates from North America. Nine A. marginale isolates derived from Canadian bison possessed identical msp4 sequences with one characteristic silent nucleotide change. The sequence of MSP1a was determined for one Canadian and two U.S. bison isolates of A. marginale, and these isolates contained 4 and 5 tandem repeats, respectively. One U.S. bison isolate tested for infectivity proved to be infective for cattle and transmitted by Dermacentor variabilis ticks. the results of this study demonstrated that these A. marginale isolates obtained from bison were similar to ones derived from naturally infected cattle.
Key Words: Anaplasma marginale • bison • ticks • cattle • major surface protein
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