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Characterization of Cellular Uptake and Distribution of Vitamin E
Human Stress Signal Research Center, National Institute of Advanced Industrial Science and Technology, Ikeda, Osaka 563-8577, Japan
Address for correspondence: Yoshiro Saito, Ph.D., Human Stress Signal Research Center, National Institute of Advanced Industrial Science and Technology, 1-8-31 Midorigaoka, Ikeda, Osaka 563-8577 Japan. Voice: +81-72-751-8293; fax: +81-72-751-9964 yoshiro-saito{at}aist.go.jp
We previously reported that tocotrienols acted as more potent inhibitors against selenium deficiency-induced cell death than the corresponding tocopherol isoforms (J. Biol. Chem. 2003;278:39428-39434). In the present study, we first compared the differences in the cellular uptake between
 -tocopherol (-Toc) and -tocotrienol (-Toc-3). The initial rate of cellular uptake of -Toc-3 was 70-fold higher than that of -Toc. Subcellular fractionation analysis of -Toc-3 and -Toc-fortified cells showed similar cellular distribution of these antioxidants, which was directly proportional to the lipid distribution. The cells containing similar amounts of -Toc-3 and -Toc showed similar resistance against the oxidative stress caused by peroxides. These results suggest that the apparent higher cytoprotective effect of -Toc-3 than -Toc is primarily ascribed to its higher cellular uptake.
Key Words: vitamin E • tocotrienol • cellular uptake • oxidative stress • antioxidant This article has been cited by other articles:
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