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Vitamin E and Gene Expression in Immune Cells
aNutritional Immunology Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University, Boston, Massachusetts 02111, USA bDepartment of Genetics and Medical Genetics, University of Wisconsin, Madison, Wisconsin, USA cNutrition and Genomics Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University, Boston, Massachusetts, USA
Address for correspondence: Sung Nim Han, Ph.D., R.D., or Simin Nikbin Meydani, DVM, Ph.D., Nutritional Immunology Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University, 711 Washington Street, Boston, MA 02111. Voice: 617-556-3242 (Han) or 617-556-3129 (Meydani); fax: 617-556-3224. sungnim.han{at}tufts.edu or simin.meydani{at}tufts.edu dCurrent address: Nestlé Research Center, Lausanne, Switzerland. eCurrent address: Department of Biology, University of North Carolina, Chapel Hill, North Carolina, USA.
Aging is associated with dysregulation of immune cells, particularly T cells. Previous studies indicated that vitamin E improves T cell function, in part by a direct effect on T cells. We studied gene expression profile of T cells to better understand the underlying mechanisms of aging- and vitamin E-induced changes in T cell function. Young and old C57BL mice were fed diets containing 30 (control) or 500 (E) ppm of vitamin E for 4 weeks. T cells were purified from splenocytes by negative selection using magnetic beads (anti-Mac-1 and anti-MHC class II), then cultured with media or stimulated with anti-CD3 and anti-CD28. Gene expression profile was assessed using microarray analysis. Genes showing more than two-fold changes, P < 0.05 by ANOVA, and with at least one present call were selected. Aging had significant effects on genes involved in signal transduction, transcriptional regulation, and apoptosis pathways in T cells, while vitamin E had a significant effect on genes associated with the regulation of cell cycle.
Key Words: vitamin E • gene expression • immune cells • aging
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