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Relaxin Stimulates cAMP Production in MCF-7 Cells upon Overexpression of Type V Adenylyl Cyclase
Department of Integrative Biology and Pharmacology, University of Texas Health Science Center, Houston, Texas 77030, USA
Address for correspondence: Dr. Carmen W. Dessauer, Department of Integrative Biology and Pharmacology, University of Texas Health Science Center, Houston, TX 77030. Voice: 713-500-6308; fax: 713-500-7444. carmen.w.dessauer{at}uth.tmc.edu
Relaxin stimulates cAMP production and activation of ERK and PI3K in THP-1 cells. Relaxin also stimulates protein kinase C zeta (PKC
 ) translocation to the plasma membrane in a PI3K-dependent manner in THP-1 and MCF-7 cells. However, relaxin did not increase cAMP production in MCF-7 cells. We overexpressed different adenylyl cyclase (AC) isoforms in MCF-7 cells to examine coupling of endogenous relaxin receptors to cAMP production. Overexpression of types II and IV AC had no effect on cAMP production by relaxin. However, overexpression of type V AC, which is activated by PKC, showed synergistic stimulation of cAMP by relaxin and forskolin.
Key Words: adenylyl cyclase • PI3K • PKC • relaxin • cyclic AMP • ACV • ACIIThis article has been cited by other articles:
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