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Dissecting the Rickettsia prowazekii Genome: Genetic and Proteomic Approaches
aDepartment of Microbiology and Immunology, Laboratory of Molecular Biology, and bProteomics and Mass Spectrometry, Cancer Research Institute, University of South Alabama College of Medicine, Mobile, Alabama 36688, USA
Address for correspondence: Dr. David Wood, Department of Microbiology and Immunology, Laboratory of Molecular Biology, University of South Alabama, Mobile, AL 36688. Voice: 251-460-6324; fax: 251-460-7269. dowood{at}jaguar1.usouthal.edu
The obligate nature of Rickettsia prowazekii intracellular growth places severe restrictions on the analysis of rickettsial gene function and gene expression. Fortunately, this situation is improving as methods for the genetic manipulation and proteomic analysis of this fascinating human pathogen become available. In this paper, we review the current status of rickettsial genetics and the isolation of rickettsial mutants using a genetic approach. In addition, the examination of rickettsial gene expression through characterization of the rickettsial proteome will be described. This will include a description of a high-throughput, accurate mass approach that has identified 596 rickettsial proteins in a complex rickettsial protein sample.
Key Words: Rickettsia prowazekii • genetics • proteomics This article has been cited by other articles:
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