|
 |
|||||||||||||||||||
|
|||||||||||||||||||
|
|||||||||||||||||||
 |
 |
|
|
Respiration and Life-Span Control in the Aging Model Podospora anserina
a Physical Biochemistry, Department of Chemistry, Darmstadt University of Technology, Petersenstraße 22, D-64287 Darmstadt, Germany b Botanical Institute, Johann Wolfgang Goethe-University, Marie-Curie-Strasse 9, D-60439 Frankfurt am Main, Germany
Key Words: aging • alternative oxidase • blue-native electrophoresis • colorless-native electrophoresis • complex I • complex III • cytochrome oxidase • mitochondria • oxidative phosphorylation • respirasome • supercomplex
Address for correspondence: Frank Krause, Physical Biochemistry, Department of Chemistry, Darmstadt University of Technology, Petersenstraße 22, D-64287 Darmstadt, Germany. Voice: +49 6151/165376; fax: +49 6151/164171. e-mail: f_krause{at}pop.tu-darmstadt.de http://www.tu-darmstadt.de/fb/ch/Fachgebiete/BC/AKDencher/index_en.html
Recent biochemical evidence has indicated the existence of respiratory supercomplexes as well as ATP synthase oligomers in the inner mitochondrial membrane of different eukaryotes. We have studied the organization of the respiratory chain of a wild-type strain and of two long-lived mutants of the filamentous fungus Podospora anserina. This aging model is able to respire by either the standard or the alternative pathway. In the latter, electrons are directly transferred from ubiquinol to the alternative oxidase (AOX) and thus bypass complexes III and IV. We showed that the two pathways are composed of distinct respiratory supercomplexes. These data are of significance for the understanding of both respiratory pathways as well as of life-span control and aging.
|
 |
 |
