Validation of a Rickettsia prowazekii-Specific Quantitative Real-Time PCR Cassette and DNA Extraction Protocols Using Experimentally Infected Lice

doi:10.1196/annals.1374.036

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Century of Rickettsiology: Emerging, Reemerging Rickettsioses, Molecular Diagnostics, and Emerging Veterinary Rickettsioses Volume 1078 published October 2006
Ann. N.Y. Acad. Sci. 1078: 617–619 (2006). doi: 10.1196/annals.1374.036
Copyright © 2006 by the New York Academy of Sciences
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Part V. Isolation, Cell Culture, and Diagnostics
Validation of a Rickettsia prowazekii–Specific Quantitative Real-Time PCR Cassette and DNA Extraction Protocols Using Experimentally Infected Lice

PATRICK J ROZMAJZL^a, LINDA HOUHAMDI^b, JU JIANG^a, DIDIER RAOULT^b AND ALLEN L RICHARDS^a

^{^a} Naval Medical Research Center, Silver Spring, Maryland, USA ^{^b} Faculte de Medecine – Timone, Marseille, France

Key Words: PCR studies in lice • DNA extraction in lice • Rickettsia prowazekii

Address for correspondence: Patrick J. Rozmajzl, NMRC-IDD/RDD, 503 Robert Grant Ave., Silver Spring, MD 20910-7500. e-mail: RozmajzlP{at}nmrc.navy.mil

The presence of R. prowazekii in lice using R. prowazekii-specificqPCR cassettes showed the changes of the rickettsial burdenin the lice post-infection.