 | CIRCULATING NUCLEIC ACIDS IN PLASMA OR SERUM II
Copyright © 2001 by the New York Academy of Sciences
description
Annals of the New York Academy of Sciences 945:195-206 (2001)
© 2001 New York Academy of Sciences
Hepatitis B Virus Nucleic Acids Circulating in the Blood
Distinct Patterns in HBs Carriers with Hepatocellular Carcinoma
RAOUL BREITKREUTZa,b,
WEI ZHANGa,
MIEUN LEEa,
ARMIN HOFFMANNa,
MEHMET TOKUSb,
QIN SUa,c AND
CLAUS H. SCHRÖDERa
aDepartment of Virus-Host Interactions, Deutsches Krebsforschungszentrum, Heidelberg, Germany
bI. Medizinische Klinik, Universitatsklinikum Mannheim, Universitat Heidelberg, Heidelberg, Germany
cDepartment of Pathology, Tangdu Hospital, The Fourth Military Medical University, Xi'an, China
Address for correspondence: Prof. Dr. Claus H. Schröder, Deutsches Krebsforschungszentrum, Department of Virus-Host Interactions (F0600), Im Neuenheimer Feld 242, 69120 Heidelberg, Germany. Voice: +49-6221-42 4850; fax: +49-6221-42 4852.
c.schroeder{at}dkfz.de
Abbreviations: ALT, alanine transaminase; Dig, digoxigenin; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; HBc, hepatitis B core antigen; HBe, hepatitis B e antigen; HBs, hepatitis B surface antigen; HBV, hepatitis B virus; HBx, hepatitis B virus X protein; HCC, hepatocellular carcinoma; HCV, hepatitis C virus; PCR, polymerase chain reaction; RT, reverse transcription; fRNA, full-length HBV RNA; trRNA, truncated HBV RNA; HIV, human immunodeficiency virus.
Circulating nucleic acids in serum and plasma are common in a variety of disease conditions. Here, we focus (i) on our approach for the detection of various hepatitis B virus (HBV)-related nucleic acids in liver tissue and in serum, (ii) on the progression of the chronic HBV infection, (iii) on the relation of HBV-specific nucleic acids circulating in the blood of patients with hepatocellular carcinoma, and in general (iv) on the diagnostic potential of circulating HBV nucleic acids.
Key Words: Hepatocellular carcinoma • Hepatitis B virus transcripts • Chronic infection stages • Serum RNA
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