 | CIRCULATING NUCLEIC ACIDS IN PLASMA OR SERUM II
Copyright © 2001 by the New York Academy of Sciences
description
Annals of the New York Academy of Sciences 945:239-249 (2001)
© 2001 New York Academy of Sciences
Circulating Nucleic Acids and Apoptosis
ANATOLY V. LICHTENSTEINa,
HOVSEP S. MELKONYANb,
L. DAVID TOMEIb AND
SAMUIL R. UMANSKYb
aCancer Research Center, Moscow, Russia
bXenomics Incorporated, Richmond, California, USA
Address for correspondence: Samuil R. Umansky, Xenomics Inc., 6034 Monterey Avenue, Richmond, CA 94805. Voice: 510-234-8045; fax: 510-235-6252.
sumansky{at}xenomics.net
It is well documented that plasma contains DNA from tissues throughout the body, including developing fetuses, and tumors. A portion of this DNA crosses the kidney barrier and appears in urine (i.e., transrenal DNA). However, molecular, cellular, and physiological mechanisms of the circulating DNA phenomenon and renal clearance are in an early phase of investigation. Here, we discuss possible forms of circulating DNA, factors affecting representation of different tissues and genomic sequences in plasma DNA, possible mechanisms of renal DNA clearance, and technical problems encountered in DNA isolation from urine. We suggest that apoptotic cells are an important source of DNA in both plasma and urine. Further analysis of the data has led us to propose that a significant portion of circulating DNA can be represented in apoptotic bodies.
Key Words: Circulating DNA • Transrenal DNA • Blood • Plasma • Serum • Urine • Apoptosis • Apoptotic bodies • Serum amyloid P component (SAP)
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