 | CIRCULATING NUCLEIC ACIDS IN PLASMA OR SERUM II
Copyright © 2001 by the New York Academy of Sciences
description
Annals of the New York Academy of Sciences 945:250-257 (2001)
© 2001 New York Academy of Sciences
Cell-Free DNA in Urine
A Marker for Kidney Graft Rejection, but Not for Prenatal Diagnosis?
XIAO YAN ZHONGa,
DEIRDRÉ HAHNb,
CAROLYN TROEGERa,
ANDRÉ KLEMMc,
GÜNTER STEINc,
PETER THOMSONb,
WOLFGANG HOLZGREVEa AND
SINUHE HAHNa
aDepartment of Obstetrics and Gynecology, University of Basel, CH 4031 Basel, Switzerland
bDepartment of Pediatric Nephrology, Wits University, Johannesburg, South Africa
cDivision of Nephrology, University of Jena, Jena, Germany
Address for correspondence: Dr. Sinuhe Hahn, Laboratory for Prenatal Medicine, Department of Obstetrics and Gynecology, University of Basel, Schanzenstrasse 46, CH 4031 Basel, Switzerland. Voice: +41 61 325 9224/9595; fax: +41 61 325 9399.
shahn{at}uhbs.ch
Intrigued by the rapid clearance of free fetal DNA from the maternal circulation, we have investigated whether this fetal genetic material could be cleared via the kidney. For this purpose, we examined for the presence of Y chromosome-specific DNA sequences in urine samples obtained from 8 women pregnant with male fetuses. No male-specific sequences could be detected, despite the use of a very sensitive nested PCR assay nor a highly reproducible real-time PCR assay. We did, however, detect maternal DNA sequences. To determine if this cell-free DNA was derived from the kidney or another source, we next examined urine from female kidney transplant patients who had received male kidneys. Y chromosome-specific sequences were indeed detectable by both nested and real-time PCR in these samples, thereby confirming a recent report describing urinary DNA microchimerism. Quantitative analysis of serially obtained samples furthermore suggests that transplant-derived sequences are elevated during periods of graft rejection. These results imply that the measurement of graft-derived urinary DNA may serve as a new marker for kidney graft tolerance.
Key Words: Cell-free DNA • Transplant • Graft rejection
This article has been cited by other articles:
|
|
|
|
 
Y. LI, D. HAHN, F. WENZEL, W. HOLZGREVE, and S. HAHN
Detection of SNPs in the Plasma of Pregnant Women and in the Urine of Kidney Transplant Recipients by Mass Spectrometry.
Ann. N.Y. Acad. Sci.,
September 1, 2006;
1075:
144 - 147.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Lazar, B. Nagy, Z. Ban, G. R Nagy, and Z. Papp
Presence of Cell-Free Fetal DNA in Plasma of Women with Ectopic Pregnancies,
Clin. Chem.,
August 1, 2006;
52(8):
1599 - 1601.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Li, D. Hahn, W. Holzgreve, and S. Hahn
Ready Detection of Donor-Specific Single-Nucleotide Polymorphisms in the Urine of Renal Transplant Recipients by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry
Clin. Chem.,
October 1, 2005;
51(10):
1903 - 1904.
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Li, X. Y. Zhong, A. Kang, C. Troeger, W. Holzgreve, and S. Hahn
Inability to Detect Cell Free Fetal DNA in the Urine of Normal Pregnant Women nor in Those Affected by Preeclampsia Associated HELLP Syndrome
Reproductive Sciences,
December 1, 2003;
10(8):
503 - 508.
[Abstract]
[PDF]
|
|
|
|
|
|
|
C. Stemmer, M. Beau-Faller, E. Pencreac'h, E. Guerin, A. Schneider, D. Jaqmin, E. Quoix, M.-P. Gaub, and P. Oudet
Use of Magnetic Beads for Plasma Cell-free DNA Extraction: Toward Automation of Plasma DNA Analysis for Molecular Diagnostics
Clin. Chem.,
November 1, 2003;
49(11):
1953 - 1955.
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Li, D. Hahn, X. Y. Zhong, P. D. Thomson, W. Holzgreve, and S. Hahn
Detection of Donor-specific DNA Polymorphisms in the Urine of Renal Transplant Recipients
Clin. Chem.,
April 1, 2003;
49(4):
655 - 658.
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Y.N. Lui, K.-S. Woo, A. Y.M. Wang, C.-K. Yeung, P. K.T. Li, E. Chau, P. Ruygrok, and Y.M. D. Lo
Origin of Plasma Cell-free DNA after Solid Organ Transplantation
Clin. Chem.,
March 1, 2003;
49(3):
495 - 496.
[Full Text]
[PDF]
|
|
|
|
