 | CIRCULATING NUCLEIC ACIDS IN PLASMA OR SERUM II
Copyright © 2001 by the New York Academy of Sciences
description
Annals of the New York Academy of Sciences 945:93-102 (2001)
© 2001 New York Academy of Sciences
Circulating Nucleosomes in Serum
STEFAN HOLDENRIEDERa,
PETRA STIEBERa,
HEINZ BODENMÜLLERb,
MARTIN BUSCHc,
JOACHIM VON PAWELd,
ANDREAS SCHALHORNe,
DOROTHEA NAGELa AND
DIETRICH SEIDELa
aInstitute of Clinical Chemistry, Klinikum der Universität München-Großhadern, Munich, Germany
bRoche Diagnostics, Penzberg, Germany
cDepartment of Radiotherapy and Radio-Oncology, Klinikum der Universität München-Großhadern, Munich, Germany
dDepartment of Oncology, Asklepios Fachkliniken, Gauting, Germany
eDepartment of Internal Medicine III, Klinikum der Universität München-Großhadern, Munich, Germany
Address for correspondence: Dr. med. Petra Stieber, Institute of Clinical Chemistry, Klinikum der Universität München-Großhadern, Marchioninistrasse 15, D-81377 München, Germany. Voice: 0049-89-7095 3115; fax: 0049-89-7095 6298.
stieber{at}klch.med.uni-muenchen.de
In the nucleus of eukaryotic cells, DNA is associated with several protein components and forms complexes known as nucleosomes. During cell death, particularly during apoptosis, endonucleases are activated that cleave the chromatin into multiple oligo- and mononucleosomes. Subsequently, these nucleosomes are packed into apoptotic bodies and are engulfed by macrophages or neighboring cells. In cases of high rates of cellular turnover and cell death, they also are released into the circulation and can be detected in serum or plasma. As enhanced cell death occurs under various pathologic conditions, elevated amounts of circulating nucleosomes are not specific for any benign or malignant disorder. However, the course of change in the nucleosomal levels in circulation of patients with malignant tumors during chemotherapy or radiotherapy is associated with the clinical outcome and can be useful for the therapeutic monitoring and the prediction of the therapeutic efficacy.
Key Words: Nucleosomes • Serum • Cancer • Chemotherapy • Dose escalation radiotherapy
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