 | CELLULAR AND MOLECULAR PHYSIOLOGY OF SODIUM-CALCIUM EXCHANGE: PROCEEDINGS OF THE FOURTH INTERNATIONAL CONFERENCE
Copyright © 2002 by the New York Academy of Sciences
description
Annals of the New York Academy of Sciences 976:382-393 (2002)
© 2002 New York Academy of Sciences
K+-Dependent Na+/Ca2+ Exchangers in the Brain
JONATHAN LYTTONa,
XIAO-FANG LIa,
HUI DONGa AND
ALEXANDER KRAEVb
aCardiovascular Research Group, Departments of Biochemistry & Molecular Biology and Physiology & Biophysics, University of Calgary, Calgary, AB, Canada T2N 4N1
bSamuel Lunenfeld Research Institute, Mt. Sinai Hospital, Toronto, ON, Canada M5G 1X5
Address for correspondence: Jonathan Lytton, Ph.D., Department of Biochemistry & Molecular Biology, University of Calgary Health Sciences Centre, Room 2518, 3330 Hospital Drive NW, Calgary, AB, Canada T2N 4N1. Voice: 403-220-2893; fax: 403-283-4841.
jlytton{at}ucalgary.ca
Ann. N.Y. Acad. Sci. 976: 382-393 (2002).
Sodium-calcium exchange was first characterized in heart myocytes and squid axon more than 3 decades ago. Since then, it has been appreciated that functioning of the Na/Ca exchanger molecule plays a critical role in calcium homeostasis in neurons. Genome analysis indicates that Na/Ca exchangers are a superfamily encoded by 7 different genes divided into 2 groups: the Na/Ca exchangers (NCX; SLC8) and the Na/Ca+K exchangers (NCKX; SLC24). Two different NCX genes, NCX1 and NCX2, are highly expressed in brain. We recently described the widespread expression of 2 NCKX-type exchangers in brain, NCKX2 and NCKX3, and uncovered evidence for expression of another, NCKX4. The unique role that each different exchanger plays in neuronal calcium homeostasis, however, awaits further investigation. To begin exploring this central question, we examined both the expression pattern and the functional properties of the K-dependent Na/Ca exchanger isoforms expressed in brain and compared and contrasted these with NCX-type exchangers. Distinct patterns of transcript abundance, regional distribution, and developmental expression were noted for each isoform. Functional properties, including stoichiometry and the kinetic characteristics of ion binding, were determined for NCKX2 and are discussed in the context of cellular Ca 2+ signaling.
Key Words: Northern blotting • in situ hybridization • recombinant expression • stoichiometry
This article has been cited by other articles:
|
|
|
|
 
O. Cuomo, R. Gala, G. Pignataro, F. Boscia, A. Secondo, A. Scorziello, A. Pannaccione, D. Viggiano, A. Adornetto, P. Molinaro, et al.
A Critical Role for the Potassium-Dependent Sodium-Calcium Exchanger NCKX2 in Protection against Focal Ischemic Brain Damage
J. Neurosci.,
February 27, 2008;
28(9):
2053 - 2063.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Visser and J. Lytton
K+-Dependent Na+/Ca2+ Exchangers: Key Contributors to Ca2+ Signaling
Physiology,
June 1, 2007;
22(3):
185 - 192.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S.-H. LEE, M.-H. KIM, J.-Y. LEE, S. H. LEE, D. LEE, K. H. PARK, and W.-K. HO
Na+/Ca2+ Exchange and Ca2+ Homeostasis in Axon Terminals of Mammalian Central Neurons
Ann. N.Y. Acad. Sci.,
March 1, 2007;
1099(1):
396 - 412.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Dong, Y. Jiang, C. R. Triggle, X. Li, and J. Lytton
Novel role for K+-dependent Na+/Ca2+ exchangers in regulation of cytoplasmic free Ca2+ and contractility in arterial smooth muscle
Am J Physiol Heart Circ Physiol,
September 1, 2006;
291(3):
H1226 - H1235.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Scheuss, R. Yasuda, A. Sobczyk, and K. Svoboda
Nonlinear [Ca2+] Signaling in Dendrites and Spines Caused by Activity-Dependent Depression of Ca2+ Extrusion
J. Neurosci.,
August 2, 2006;
26(31):
8183 - 8194.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. V. Pulina, R. Rizzuto, M. Brini, and E. Carafoli
Inhibitory Interaction of the Plasma Membrane Na+/Ca2+ Exchangers with the 14-3-3 Proteins
J. Biol. Chem.,
July 14, 2006;
281(28):
19645 - 19654.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X.-F. Li, L. Kiedrowski, F. Tremblay, F. R. Fernandez, M. Perizzolo, R. J. Winkfein, R. W. Turner, J. S. Bains, D. E. Rancourt, and J. Lytton
Importance of K+-dependent Na+/Ca2+-exchanger 2, NCKX2, in Motor Learning and Memory
J. Biol. Chem.,
March 10, 2006;
281(10):
6273 - 6282.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Dong, Z. M. Sellers, A. Smith, J. Y. C. Chow, and K. E. Barrett
Na+/Ca2+ exchange regulates Ca2+-dependent duodenal mucosal ion transport and HCO3- secretion in mice
Am J Physiol Gastrointest Liver Physiol,
March 1, 2005;
288(3):
G457 - G465.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Zhang, J. X.-J. Yuan, K. E. Barrett, and H. Dong
Role of Na+/Ca2+ exchange in regulating cytosolic Ca2+ in cultured human pulmonary artery smooth muscle cells
Am J Physiol Cell Physiol,
February 1, 2005;
288(2):
C245 - C252.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Annunziato, G. Pignataro, and G. F. Di Renzo
Pharmacology of Brain Na+/Ca2+ Exchanger: From Molecular Biology to Therapeutic Perspectives
Pharmacol. Rev.,
December 1, 2004;
56(4):
633 - 654.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Cai and J. Lytton
The Cation/Ca2+ Exchanger Superfamily: Phylogenetic Analysis and Structural Implications
Mol. Biol. Evol.,
September 1, 2004;
21(9):
1692 - 1703.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Cai and J. Lytton
Molecular Cloning of a Sixth Member of the K+-dependent Na+/Ca2+ Exchanger Gene Family, NCKX6
J. Biol. Chem.,
February 13, 2004;
279(7):
5867 - 5876.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Lencesova, A. O'Neill, W. G. Resneck, R. J. Bloch, and M. P. Blaustein
Plasma Membrane-Cytoskeleton-Endoplasmic Reticulum Complexes in Neurons and Astrocytes
J. Biol. Chem.,
January 23, 2004;
279(4):
2885 - 2893.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
