 | RICKETTSIOLOGY: Present and Future Directions
Copyright © 2003 by the New York Academy of Sciences
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Annals of the New York Academy of Sciences 990:302-310 (2003)
© 2003 New York Academy of Sciences
Real-Time PCR Duplex Assay for Rickettsia prowazekii and Borrelia recurrentis
JU JIANGa,b,
JOSEPH J. TEMENAKa,c AND
ALLEN L. RICHARDSa,d
aNaval Medical Research Center, Silver Spring, Maryland 20910-7500, USA
bHenry M. Jackson Foundation, Rockville, Maryland, USA
cFood and Drug Administration, Rockville, Maryland, USA
dUniformed Services University, Bethesda, Maryland, USA
Address for correspondence: A.L. Richards, Ph.D., Director, Rickettsial Diseases Department, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910-7500. Voice: 301-319-7668; fax: 301-319-7460.
RichardsA{at}nmrc.navy.mil
Ann. N.Y. Acad. Sci. 990: 302-310 (2003).
Rickettsia prowazekii, the etiologic agent for epidemic typhus, and Borrelia recurrentis, the etiologic agent of relapsing fever, both utilize the same vector, the human body louse ( Pediculus humanus), to transmit human disease. We have developed an assay to detect both bacterial pathogens in a single tube utilizing real-time PCR. Assays for both agents are specific. The R. prowazekii and B. recurrentis assays do not detect nucleic acid from R. typhi, R. canada, or any of eight spotted fever rickettsiae. In addition they did not react with Neorickettsia risticii, N. sennetsu, Franciscella persica, Bartonella quintana, Legionella pneumophila, Proteus mirabilis, Salmonella enterica, Escherichia coli, and Staphylococcus aureus. Moreover, the B. recurrentis assay did not detect B. duttonii, B. coriaceae, B. afzelii, B. garinii, B. hermsii, or B. burgdorferi nucleic acid. Both assays detected repeatedly only R. prowazekii or B. recurrentis either when tested alone or together in one test tube.
Key Words: typhus • Rickettsia prowazekii • Borrelia recurrentis • human body louse
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