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Ann. N.Y. Acad. Sci., Annals PrePrint, published online ahead of print December 20, 2007 doi: 10.1196/annals.1430.001 Copyright © 2007 by the New York Academy of Sciences description
1 Institute for Organic Chemistry, University of Regensburg, Universitätsstr. 31, Regensburg, D-93053, Germany; , Germany
* To whom correspondence should be addressed. E-mail: achim.wagenknecht{at}chemie.uni-regensburg.de. PrePrint Abstract
There is an increasing need for fluorescent nucleic acid probes that are able to sense genetic variations without the application of enzymes. The incorporation of organic fluorophores either as DNA base modifications or as DNA base substitutions represent a powerful and versatile method for such new fluorescent DNA assays. Multiple labeling of oligonucleotides using several adjacent chromophore-modified DNA bases yield fluorescence enhancement and modulation that are sensitive to single base mismatches in the complementary oligonucleotide. Charge transfer processes that cause fluorescence quenching are DNA-base mediated and occur over several base pairs distance. Our 'DETEQ' setup consisting of a fluorescence DNA base substitution and the charge acceptor as a second modification three base pairs away allows the homogeneous detection of single base mutations simply by fluorescence readout. This could lead to new DNA microarrays which are based on charge transfer processes and can be analyzed by commonly used fluorescence readout techniques. Key Words:
Chromophore, DNA, Electron Transfer, Fluorescence
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